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Probing the intracellular fate of supramolecular nanocarriers and their cargo with FRET schemes

Callan, J, Callan, Bridgeen, Fowley, Colin, Thapaliya, Ek Raj, Tang, S.C, Zhang, Y and Raymo, Francisco (2017) Probing the intracellular fate of supramolecular nanocarriers and their cargo with FRET schemes. In: COLLOIDAL NANOPARTICLES FOR BIOMEDICAL APPLICATIONS XII. Proceeding of SPIE. SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA, UNSP 100781B-UNSP 100781B. ISBN ISBN-10: 1510605975 [Book section]

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Abstract

We designed a strategy to monitor self-assembling supramolecular nanocarriers and their cargo simultaneously in the intracellular space with fluorescence measurements. It is based on Forster resonance energy transfer (FRET) between complementary chromophores covalently integrated in the macromolecular backbone of amphiphilic polymers and/or noncovalently encapsulated in supramolecular assemblies of the amphiphilic components. Indeed, these polymers assemble into a micelles in aqueous phase to bring energy donors and acceptors in close proximity and allow energy transfer. The resulting supramolecular assemblies maintain their integrity after travelling into the intracellular space and do not lose their molecular guests in the process. Furthermore, this mechanism can also be exploited to probe the fate of complementary nanoparticles introduced within cells in consecutive incubation steps. Efficient energy transfer occurs in the intracellular space after the sequential incubation of nanocarriers incorporating donors first and then nanoparticles containing acceptors or vice versa. The two sets of nanostructured assemblies ultimately co-localize in the cell interior to bring donors and acceptors together and enable energy transfer. Thus, this protocol is particularly valuable to monitor the transport properties of supramolecular nanocarriers inside living cells and can eventually contribute to the fundamental understating of the ability of these promising vehicles to deliver contrast agents and/or drugs intracellularly in view of possible diagnostics and/or therapeutic applications

Item Type:Book section
Keywords:Amphiphilic polymers; nanoparticles; self-assembly; nanocarriers; energy transfer; fluorescence imaging
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:39965
Deposited By: Professor John Callan
Deposited On:21 Apr 2018 15:18
Last Modified:21 Apr 2018 15:18

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