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Real time confocal laser scanning microscopy: Potential applications in space medicine and cell biology

Rollan, A, Ward, T and McHale, Anthony (1998) Real time confocal laser scanning microscopy: Potential applications in space medicine and cell biology. Acta Astronautica, 42 (1-8, S). pp. 37-50. [Journal article]

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Photodynamic therapy (PDT) in which tissues may be rendered fatally light-sensitive represents a relatively novel treatment for cancer and other disorders such as cardiovascular disease. It offers significant application to disease control in an isolated environment such as space flight. In studying PDT in the laboratory, low energy lasers such as HeNe lasers are used to activate the photosensitized cellular target. A major problem associated with these studies is that events occurring during actual exposure of. the target cells to the system cannot be examined in real time. In this study HeLa cells were photosensitized and photodynamic activation was accomplished using the scanning microbeam om a confocal laser scanning microscope. This form of activation allowed for simultaneous photoactivation and observation and facilitated the recording of events at a microscopic level during photoactivation. Effects of photodynamic activation on the target cells were monitored using the fluorophores rhodamine 123 and ethidium homodimer-1. Potential applications of these forms of analyses to space medicine and cell biology are discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:3997
Deposited By: Professor Anthony McHale
Deposited On:18 Dec 2009 11:25
Last Modified:11 Mar 2015 12:29

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