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Luminescent Detection of ATP in Aqueous Solution Using Positively Charged CdSe-ZnS Quantum Dots

Callan, John F., Mulrooney, Ray C. and Karnila, Sukanta (2008) Luminescent Detection of ATP in Aqueous Solution Using Positively Charged CdSe-ZnS Quantum Dots. JOURNAL OF FLUORESCENCE, 18 (6). pp. 1157-1161. [Journal article]

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DOI: 10.1007/s10895-008-0367-5


Commercially available CdSe-ZnS Quantum Dots (QDs) have been modified by exchanging the hydrophobic surface ligands with (2-mercaptoethyl)-trimethylammonium chloride. The resulting water soluble conjugate was titrated with solutions of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate, guanosine triphosphate (GTP), guanosine diphosphate and guanosine monophosphate in 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.4). A strong fluorescence quench of about 80% was observed for ATP, a quench of 25% was observed for GTP while the others had virtually no effect. The quenching effect of ATP and GTP was attributed to the high negative charge density associated with these substrate's resulting in a strong attraction to the QD surface enabling them to engage in electron transfer with the excited QD. The lack of fluorescence quenching associated with the other nucleotides was most likely due to their reduced charge density resulting in a lower affinity for the QD surface.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:4064
Deposited By: Professor John Callan
Deposited On:05 Jan 2010 14:05
Last Modified:19 Nov 2012 15:25

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