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Biosurfactant Production by Azotobacter chroococcum Isolated from the Marine Environment

Thavasi, R., Nambaru, V. R. M. Subramanyam, Jayalakshmi, S., Balasubramanian, T. and Banat, Ibrahim (2009) Biosurfactant Production by Azotobacter chroococcum Isolated from the Marine Environment. MARINE BIOTECHNOLOGY, 11 (5). pp. 551-556. [Journal article]

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DOI: 10.1007/s10126-008-9162-1


Preliminary characterization of a biosurfactant-producing Azotobacter chroococcum isolated from marine environment showed maximum biomass and biosurfactant production at 120 and 132 h, respectively, at pH 8.0, 38A degrees C, and 30aEuro degrees salinity utilizing a 2% carbon substrate. It grew and produced biosurfactant on crude oil, waste motor lubricant oil, and peanut oil cake. Peanut oil cake gave the highest biosurfactant production (4.6 mg/mL) under fermentation conditions. The biosurfactant product emulsified waste motor lubricant oil, crude oil, diesel, kerosene, naphthalene, anthracene, and xylene. Preliminary characterization of the biosurfactant using biochemical, Fourier transform infrared spectroscopy, and mass spectral analysis indicated that the biosurfactant was a lipopeptide with percentage lipid and protein proportion of 31.3:68.7.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:4120
Deposited By: Professor Ibrahim Banat
Deposited On:14 Jan 2010 11:00
Last Modified:16 May 2012 10:13

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