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Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation

Aburuwaida, AS, Banat, Ibrahim, Haditirto, S, Salem, A and Kadri, M (1991) Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation. Acta Biotechnologica, 11 (4). pp. 315-324. [Journal article]

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A gram-positive, nonfermentative, rod-shaped bacterium designated St-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly glycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during, exposure to high salinity (10% NaCl), elevated temperatures (120-degrees-C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86% of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:4353
Deposited By: Professor Ibrahim Banat
Deposited On:07 Jan 2010 19:22
Last Modified:11 Mar 2015 14:53

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