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Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials

Rahman, K. S., Rahman, T. J., McClean, Stephen, Marchant, R and Banat, Ibrahim (2002) Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. Biotechnology progress, 18 (6). pp. 1277-1281. [Journal article]

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This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C(10)-C(10)). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.

Item Type:Journal article
Keywords:Emulsions; Glycerol/metabolism; Glycolipids/biosynthesis/chemistry/economics; Hydrocarbons; Mass Spectrometry; Pseudomonas aeruginosa/chemistry/metabolism; Safflower Oil/metabolism; Soybean Oil/metabolism; Surface-Active Agents/economics
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
Biomedical Sciences Research Institute > Diabetes
ID Code:5086
Deposited By: Prof Stephen McClean
Deposited On:06 Jan 2010 16:18
Last Modified:24 Jun 2015 11:10

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