Brady, D, Singh - Nee Nigam, Poonam, Marchant, Roger and McHale, Anthony (1997) Ethanol production at 45 degrees C by alginate-immobilized Kluyveromyces marxianus IMB3 during growth on lactose-containing media. BIOPROCESS ENGINEERING, 16 (2). pp. 101-104. [Journal article]
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The thermotolerant yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and this was used in batch-fed reactor systems to convert lactose (4 g/l) to ethanol. Production of ethanol by the free and immobilized biocatalyst in the presence and absence of Mn2+ was compared. In systems containing the free microorganism in the presence and absence of Mn2+, ethanol increased to a maxi mum of 8 g/l within 40 hours with no significant difference in production by both systems. Ethanol production by the immobilized system in the absence of Mn2+ increased to a maximum of 13 g/l within 40 hours and then decreased to 9 g/l within 80 hours. Ethanol production by the immobilized system in the presence of Mn2+ increased to 14 g/l within 60 hours and this decreased to 13 g/l at 80 hours. When all systems were re-fed at 80 hours, ethanol production by systems containing the free biocatalyst increased to a maximum of 3 g/l while the immobilized system in the presence of Mn2+ increased to a maximum of 12 g/l. Subsequent experiments involving re-feeding the system at shorter time intervals demonstrated that ethanol production by the immobilized system on lactose-containing media at 45 degrees C was far superior to ethanol production by the free biocatalyst.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
|Deposited By:||Dr Poonam Singh - Nee Nigam|
|Deposited On:||21 Dec 2009 11:24|
|Last Modified:||04 Dec 2012 11:42|
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