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The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity

Hughes, Ciara, Lewis, SEM, McKelvey-Martin, Valerie and Thompson, W (1998) The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. HUMAN REPRODUCTION, 13 (5). pp. 1240-1247. [Journal article]

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The integrity of sperm DNA is crucial for the maintenance of genetic health. A major source of damage is reactive oxygen species (ROS) generation; therefore, antioxidants may afford protection to sperm DNA, The objectives of the study were, first, to measure the effects of antioxidant supplementation in vitro on endogenous DNA damage in spermatozoa using the single cell gel electrophoresis (comet) assay and, second, to assess the effect of antioxidant supplementation given prior to X-ray irradiation on induced DNA damage. Spermatozoa from 150 patients were prepared by Percoll centrifugation in the presence of ascorbic acid (300, 600 mu M), alpha tocopherol (30, 60 mu M), urate (200, 400 mu M), or acetyl cysteine (5, 10 mu M). DNA damage was induced by 30 Gy X-irradiation, DNA strand breakage was measured using the comet assay. Sperm DNA was protected from DNA damage by ascorbic acid (600 mu M), alpha tocopherol (30 and 60 mu M) and urate (400 mu M), These antioxidants provided protection from subsequent DNA damage by X-ray irradiation. In contrast, acetyl cysteine or ascorbate and alpha tocopherol together induced further DNA damage. Supplementation in vitro with the antioxidants ascorbate, urate and alpha tocopherol separately has beneficial effects for sperm DNA integrity.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Health Sciences
Research Institutes and Groups:Institute of Nursing and Health Research > Centre for Health and Rehabilitation Technologies
Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:9257
Deposited By: Professor Valerie McKelvey-Martin
Deposited On:22 Jan 2010 12:27
Last Modified:04 Dec 2012 12:30

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