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Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

Bigot, Stephane, Lucas, Luciano, Morrow, Philip, Mitchell, Christopher and Saetzler, Kurt (2007) Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In: 2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3. ISBI. 510 pp. [Conference contribution]

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The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

Item Type:Conference contribution (Paper)
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Computing and Information Engineering
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Computer Science Research Institute
Biomedical Sciences Research Institute > Genomic Medicine
Computer Science Research Institute > Information and Communication Engineering
ID Code:9467
Deposited By: Professor Christopher Mitchell
Deposited On:28 Mar 2013 15:17
Last Modified:10 May 2017 10:48

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