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Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

Bigot, Stephane, Lucas, Luciano, Morrow, Philip, Mitchell, Christopher and Saetzler, Kurt (2007) Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In: 2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3. ISBI. 510 pp. [Conference contribution]

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Abstract

The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

Item Type:Conference contribution (Paper)
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Computing and Information Engineering
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Computer Science Research Institute
Biomedical Sciences Research Institute > Genomic Medicine
Computer Science Research Institute > Information and Communication Engineering
ID Code:9467
Deposited By: Professor Christopher Mitchell
Deposited On:28 Mar 2013 15:17
Last Modified:10 May 2017 10:48

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